BioMaster HS-Taq PCR Kit contains all components needed for conventional hot-start PCR (except for DNA template and primers): hot start Taq DNA polymerase solution (5 U/µl), 5× PCR buffer, 50 mM MgCl2, 50× dNTP mix and 6× loading buffer.
5× PCR buffer (not containing MgCl2) is optimized for efficient and reproducible PCR. Its components increase stability of HS-Taq DNA polymerase during the reaction. The buffer is chemically stable, inert and its composition does not affect optimal annealing temperature or the parameters of template melting. 50 mM MgCl2 and 50× dNTP mix included in the kit allow optimizing PCR conditions for individual primer pairs, while 6× loading buffer eases sample preparation for analysis and allows monitoring the progress of electrophoresis.
|Long term storage at -20 °С. Can be shipped at ambient temperature. Avoid repetitive freeze-thaw cycles.|
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