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chb-40120-020

BioMaster HS-qPCR (2×)

BioMaster HS-qPCR (2×)
Master mix containing Hot-Start Taq DNA polymerase for real-time PCR with fluorescent probes


Price: 50.00 EUR
Not in stock

BioMaster HS-qPCR (2×) contains 2× BioMaster HS-qPCR reaction mix and sterile water. The kit was developed for quantitative real-time PCR with fluorescently labeled probes. The mix contains all components, besides DNA template, primers and probe, needed for a PCR with fluorescently labeled probes: HS-Taq DNA polymerase, dNTPs mixture, 2× PCR buffer and Mg2+.

The master mix is optimized for  efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples. The mix contains additional components increasing stability of HS-Taq DNA polymerase during the reaction. BioMaster HS-qPCR (2×) includes components that influence primer annealing temperature and characteristics of template melting thus enabling to increase PCR specificity and use templates with complicated spatial structure. 

DNA polymerase included in the kit is inactive at room temperature and requires preheating at 95 °C for 5 min. Use of the kit saves time and minimizes the risk of contamination due to reduced number of pipetting steps.

Kit content:
  • 100 mM Tris-HCl (рН 8.5 at 25 °С)
  • 100 mM KCl
  • dNTPs (0.4 mM each)  
  • 10 mM MgCl2
  • 0.1 U/µl Taq DNA polymerase
  • 0.025% Tween 20
  • stabilizers of HSTaq DNA polymerase
Applications:
  • Hot-start real-time PCR with fluorescently-labeled probes
  • Conventional PCR
  • High-throughput PCR
  • Multiplex PCR
  • Genotyping
Storage:
Long term storage at -20 °С. Can be shipped at ambient temperature. Avoid repetitive freeze-thaw cycles.
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