The BioMaster HS-qPCR Hi-ROX (2×) kit contains 2× BioMaster HS-qPCR Hi-ROX reaction mix and sterile water. The mix contains all components, besides DNA template and primers, needed for PCR: HS-Taq DNA polymerase, dNTP mix, 2× PCR buffer, Mg²⁺ solution and the ROX dye

BioMaster HS-qPCR Hi-ROX (2×) is optimized for efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples. The mix contains additional components increasing stability of HS-Taq DNA polymerase during the reaction. The components of the mix do not affect optimal annealing temperature or parameters of template melting. DNA polymerase included in the mix is inactive at room temperature and requires preheating at 95 °C for 5 min. BioMaster HS-qPCR Hi-ROX (2×) master mix is suitable for PCR platforms that use ROX passive dye as a reference guide, such as Thermo Fisher (formerly ABI) 7000, 7300, 7700, 7900, 7900HT and StepOnePlus. Use of the kit saves time and minimizes the risk of contamination due to reduced number of pipetting steps.