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chb-40520-004

BioMaster LR HS-PCR (2×)

BioMaster LR HS-PCR (2×)
The mastermix containing HS-Taq DNA polymerase and Pfu DNA polymerase for long range PCR (up to 30 kb)


Price: 70.00 EUR
Not in stock

BioMaster LR HS-PCR (2×) kit includes 2× BioMaster LR HS-PCR (2×) reaction mix, sterile water and 6× loading buffer. The master mix is developed for amplification of long (0.2 through 30 kbp) DNA fragments with enhanced specificity, high fidelity and high reaction yield. The mix is also perfectly suitable for amplification of GC-rich (>65%) and complicated DNA regions. BioMaster LR HS-PCR (2×) contains all components necessary for PCR (except for DNA template and primers): polymerase mix (HS-Taq and Pfu), dNTPs mixture, PCR buffer, and Mg2+

Reaction mix content:
  • 100 mM Tris-HCl (рН 8.9 at 25 °С)
  • 100 mM KCl
  • dNTPs (0.8 mM each)  
  • 4 mM MgSO4
  • 0.1 U/µl polymerase mix
  • 0.2% Tween 20
  • stabilizers of DNA polymerases
Applications:
  • Long-range PCR
  • Product generation for TA-cloning
  • Amplification of GC-rich and complicated templates
Storage:
Long term storage at -20 °С. Can be shipped at ambient temperature. Avoid repetitive freeze-thaw cycles.
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