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chb-40521-004

BioMaster LR HS-PCR-Color (2×)

BioMaster LR HS-PCR-Color (2×)
for hot-start amplification of long DNA fragments of 0.2-30 kbp, GC-rich and complicated regions of DNA; contains dyes


Price: 70.00 EUR
Not in stock

BioMaster LR HS-PCR-Color (2×) includes 2× BioMaster LR HS-PCR-Color (2×) mix, DMSO and sterile water. The master mix is developed for amplification of long (0.2 through 30 kbp) DNA fragments with enhanced specificity, high fidelity and high reaction yield. The mix is also perfectly suitable for amplification of GC-rich (>65%) and complicated DNA regions.

BioMaster LR HS-PCR-Color (2×) contains all the components necessary for PCR (except for DNA template and primers): polymerase mix (HS-Taq and Pfu), dNTPs mix, PCR buffer, Mg2+ and marker dyes.

The combination of polymerases several times enhances fidelity and reliability of amplification comparing to PCR with Taq polymerase alone. Synergic performance of the two enzymes allows to obtain long PCR products (up to 15 kb for genomic and up to 30 kb for viral DNA templates). The products obtained contain mostly 3´-dA ends and can be therefore further used for cloning.

The buffer is optimized for efficient performance of both polymerases and provides high reaction yield. High density of the solution and the presence of marker dyes allow direct gel loading. The dyes included in the mix do not interfere with amplicon purification by most of the known methods.

Applications:
  • Long-range PCR
  • Product generation for TA-cloning
  • Amplification of GC-rich and complicated templates
Storage:
Long term storage at -20 °С. Can be shipped at ambient temperature. Avoid repetitive freeze-thaw cycles.
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