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chb-40130-020

BioMaster UDG HS-qPCR (2×)

BioMaster UDG HS-qPCR (2×)
The master mix is developed for quantitative real-time PCR with fluorescently labeled probes. Addition of UDG prevents re-amplification of PCR carryover products


Price: 66.00 EUR
Not in stock

BioMaster UDG HS-qPCR (2×) contains 2× BioMaster UDG HS-qPCR reaction mix and sterile water. The reaction mix contains all components necessary for PCR except for DNA template, primers and probe: hot start Taq DNA polymerase, N-uracil-DNA glycosylase, dNTP mix, PCR buffer, and Mg2+.

The mix is optimized for conducting efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples. The solution includes the components that increase stability of HS-Taq DNA polymerase in the PCR. BioMaster UDG HS-qPCR (2×) does not contain components that influence primer annealing temperature or characteristics of template melting.

N-uracil-DNA glycosylase (UDG) and dUTP (in proportion with dTTP) provide protection from amplicon transfer between reaction solutions (cross-contamination). 

Applications:
  • Hot-start real-time PCR with oligonucleotide probes
  • Conventional PCR
  • High-throughput PCR
  • Multiplex PCR
  • Genotyping
Storage:
Long term storage at -20 °С. Can be shipped at ambient temperature. Avoid repetitive freeze-thaw cycles.
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