BioMaster HS-Taq PCR Master Mix (2×) includes 2× BioMaster HS-Taq PCR Master Mix reaction mix, 50 mM MgCl2 and sterile water. The kit was developed for high throughput PCR analysis. The mix contains all components, besides DNA template and primers, needed for a PCR reaction: HS-Taq DNA polymerase, dNTPs mixture, 2× PCR buffer and MgCl2.
The master mix is optimized for efficient and reproducible hot start PCR. The mix contains additional components increasing stability of HS-Taq DNA polymerase during the reaction. BioMaster HS-Taq PCR reaction mix is chemically stable, inert and does not affect optimal annealing temperature or parameters of template melting. DNA polymerase included in the kit is inactive at room temperature and requires preheating at 95 °C for 5 min. Additional MgCl2 solution allows easy optimization of the reaction mix for each individual primer/template system. Use of the kit saves time and minimizes the risk of contamination due to reduced number of pipetting steps.
| Kit content: |
- 100 mM Tris-HCl (рН 8.5 at 25 °С)
- 100 mM KCl
- dNTPs (0.4 mM each)
- 4 mM MgCl2
- 0.06 U/µl Taq DNA polymerase
- 0.2% Tween 20
- stabilizers of HSTaq DNA polymerase
|
| Applications: |
- Hot-start PCR
- High-throughput PCR
- Conventional PCR with high reproducibility
- Generation of PCR products for TA cloning
- RT-PCR
|
| Limitations: |
- The mix is not recommended for use for amplicons ˃5 kb
- The mix is not recommended for use with fluorescently labeled probes
|
| Storage: |
| Long term storage at -20 °С. Can be shipped at ambient temperature. Avoid repetitive freeze-thaw cycles. |
