BioMaster HS-Taq PCR Master Mix (2×) includes 2× BioMaster HS-Taq PCR Master Mix reaction mix, 50 mM MgCl₂ and sterile water. The kit was developed for high throughput PCR analysis. The mix contains all components, besides DNA template and primers, needed for a PCR reaction: HS-Taq DNA polymerase, dNTPs mixture, 2× PCR buffer and MgCl₂.

The master mix is optimized for efficient and reproducible hot start PCR. The mix contains additional components increasing stability of HS-Taq DNA polymerase during the reaction. BioMaster HS-Taq PCR reaction mix is chemically stable, inert and does not affect optimal annealing temperature or parameters of template melting. DNA polymerase included in the kit is inactive at room temperature and requires preheating at 95 °C for 5 min. Additional MgCl₂ solution allows easy optimization of the reaction mix for each individual primer/template system. Use of the kit saves time and minimizes the risk of contamination due to reduced number of pipetting steps.