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chb-10183

8-Oxo-2’-deoxyguanosine-5’-triphosphate (8-oxo-dGTP)

A mutagenic dNTP which can be incorporated into DNA by PCR.
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8-Oxo-2'-deoxyguanosine-5'-Triphosphate (8-Oxo-dGTP) and dPTP, are mutagenic dNTPs which can be incorporated into DNA by PCR using standard Taq polymerase. Random mutagenesis is induced by amplification of the target in the presence of the four natural dNTPs and their mutagenic analogs, 8-Oxo-dGTP and/or dPTP. When both mutagenic analogs are used, the rate of mutagenesis is determined by the number of PCR cycles. 8-Oxo-dGTP can mispair with A, leading to A-to-C and G-to-T transversion mutations, which occur in a 1 : 1.5 ratio in PCR. When dPTP is used in combination with 8-Oxo-dGTP in PCR, both transition mutations (A-to-G and G-to-A) and transversion mutations (A-to-C and G-to-T) can be produced.

Molecular Formula: C10H16N5O14P3 (free acid)

Specification:
  • HPLC Purity: > 96%
Applications:
  • Influence on base excision repair
  • Influence on erroneous incorporation by DNA-polymerases
  • Hydrolysis to 8-oxo-dGMP by E.-coli MutT
  • Triggering cell senescence through formation of ROS
Format:

10 mM solution in water.

Storage Conditions:
  • Store at -20°C. 
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