Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labelled with any amine-reactive fluorescent dye, biotin, or hapten. Dye labelled aminoallyl modified RNAs are used in microarray analysis and for localization of RNA in the cell. Also, 1,10-phenanthroline can be coupled to an aminoallyl modified RNA for sequence specific cleavage of nucleic acids.
Since AA-UTP (as well as AA-CTP) maintains a strict Watson-Crick base pairing recognition, it is suited for the systematic evolution of ligands by exponential enrichment (SELEX) process thus allowing introduction of primary amino-functionalities to RNA libraries.
Molecular formula: C12H20N3O15P3 (Acid Form)
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0.1 M solution in water. |
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