5-Aminoallyl-uridine-5'-Triphosphate (Aminoallyl-UTP)

A reagent for indirect non-radioactive enzymatic labeling of RNA during in vitro transcription

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Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labelled with any amine-reactive fluorescent dye, biotin, or hapten. Dye labelled aminoallyl modified RNAs are used in microarray analysis and for localization of RNA in the cell. Also, 1,10-phenanthroline can be coupled to an aminoallyl modified RNA for sequence specific cleavage of nucleic acids.

Since AA-UTP (as well as AA-CTP) maintains a strict Watson-Crick base pairing recognition, it is suited for the systematic evolution of ligands by exponential enrichment (SELEX) process thus allowing introduction of primary amino-functionalities to RNA libraries.

Molecular formula: C12H20N3O15P3  (Acid Form)

  • HPLC Purity: > 96%
Tested for:
  • Functionality in in vitro transcription with T7 RNA Polymerase
  • Absence of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities

0.1 M solution in water.

Storage Conditions:
  • Store at -20°C. 
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