Hot start Taq DNA Polymerase

Hot start Taq DNA Polymerase
For PCR, optimized mixture of Taq Polymerase and Anti-Taq monoclonal antibodies

Price: 70.00 EUR
Not in stock
Hot Start Taq DNA Polymerase is the optimized mixture of Taq Polymerase and Anti-Taq monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature (20-22 °C). The inhibition of Taq DNA polymerase is completely reversed when the temperature is above 70°C. The PCR products obtained with Hot Start Taq DNA Polymerase are free from unspecific products and from primer-dimers.

Hot Start Taq DNA Polymerase provides:

reliable and reproducable quantification in qPCR
perfect performance in real time PCR
reaction set-up at room temperature
activation of enzyme during first heating
no necessity for changes or optimization of the protocol 
high specificity, reduced primer mismatch or dimers

A 5 U/µl solution of the enzyme in a storage buffer:

10 mM Tris-HCl (pH 7.0)
50 mM KCl
0.1 mM EDTA
50% glycerol

Unit definition:
One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C.
Hot start PCR
Real time PCR
Amplification of complex genomic and cDNA templates
Multiplex PCR
High specifity PCR
Reaction buffer:
160 mM (NH4)2SO4
670 mM Tris-HCl (pH 8,8 at 25°C)
2.5 mM MgCl2
Storage Conditions:
Store at -20°C. Can be shipped on ice packs.
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