Login

chb-20006-003

Hot start Taq DNA Polymerase

Hot start Taq DNA Polymerase
For PCR, optimized mixture of Taq Polymerase and Anti-Taq monoclonal antibodies


Price: 70.00 EUR
Not in stock
Features:
Hot Start Taq DNA Polymerase is the optimized mixture of Taq Polymerase and Anti-Taq monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature (20-22 °C). The inhibition of Taq DNA polymerase is completely reversed when the temperature is above 70°C. The PCR products obtained with Hot Start Taq DNA Polymerase are free from unspecific products and from primer-dimers.

Hot Start Taq DNA Polymerase provides:

reliable and reproducable quantification in qPCR
perfect performance in real time PCR
reaction set-up at room temperature
activation of enzyme during first heating
no necessity for changes or optimization of the protocol 
high specificity, reduced primer mismatch or dimers

Format:
A 5 U/µl solution of the enzyme in a storage buffer:

10 mM Tris-HCl (pH 7.0)
50 mM KCl
0.1 mM EDTA
50% glycerol

Unit definition:
One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C.
Applications:
Hot start PCR
Real time PCR
Amplification of complex genomic and cDNA templates
Multiplex PCR
High specifity PCR
Reaction buffer:
160 mM (NH4)2SO4
670 mM Tris-HCl (pH 8,8 at 25°C)
0,1%Tween-20
2.5 mM MgCl2
Storage Conditions:
Store at -20°C. Can be shipped on ice packs.
Copyright MAXXmarketing GmbH
JoomShopping Download & Support

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.

Ok