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chb-40700-001

Reverse transcriptase M-MuLV–RH Kit

Reverse transcriptase M-MuLV–RH Kit
consists of M-MuLV–RH revertase and 5× RT buffer mix


Price: 60.00 EUR
Not in stock

Reverse transcriptase M-MuLV–RH Kit consists of M-MuLV–RH revertase and 5× RT buffer mix containing all components needed for revertase action (except for RNA template and primers). The buffer is optimized for efficient reaction of reverse transcription across a wide range of RNA templates.

The enzyme:
M-MuLV–RH is a genetically modified reverse transcriptase (revertase) from murine leukemia virus (M-MuLV) that is different from wild-type M-MuLV in structure, catalytic features and temperature optimum of activity. The enzyme possesses RNA- and DNA-dependent polymerase activity but lacks RNase H activity. Temperature optimum for M-MuLV-RH activity is 42°C (the enzyme remains active at temperatures up to 50 °C). The enzyme is able to synthesize first strand cDNA up to 9 kb and incorporate modified bases.
Applications:
  • First strand cDNA synthesis for RT-PCR and real-time RT-PCR
  • cDNA generation for TA-cloning
  • Generation of labeled cDNA probes for microarrays
  • DNA labeling
  • RNA analysis by primer extension
Storage:
Long term storage at -20 °С. Can be shipped at ambient temperature. Avoid repetitive freeze-thaw cycles.
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