M-MuLV–RH is a genetically modified reverse transcriptase (revertase) from murine leukemia virus (M-MuLV) that is different from wild-type M-MuLV in structure, catalytic features and temperature optimum of activity. The enzyme possesses RNA- and DNA-dependent polymerase activity but lacks RNase H activity. Temperature optimum for M-MuLV-RH activity is 42°C (the enzyme remains active at temperatures up to 50 °C). The enzyme is able to synthesize first strand cDNA up to 9 kb and incorporate modified bases.
|400,000 units/ml in storage buffer (50 mM TrisHCl, (pH 8,3), 100mM NaCl, 1mM EDTA, 0,1 mM DTT, 0,1%Triton X-100, 50% glycerol)|
|One unit of activity is the amount of enzyme required to incorporate 1 nmole of dTTP into an acid-insoluble form in 10 minutes at 37°C using polyA-oligo(dT) as template and primer.|
|Recommended reaction buffer for RT-PCR:|
|50 mM Tris-HCl (pH 8.3 at 25°C), 2-8 mM MgCl2, 10 mM DTT, 100 mM KCl. Optional: 2-4 mM MnCl2.|
|Store at -20°C.|
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