T7 RNA Polymerase

T7 RNA Polymerase
Recombinant. Incorporates modified (e.g. labeled) nucleotides.

Price: 75.00 EUR
Not in stock
T7 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for its respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. Incorporates modified (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled) nucleotides.
Unit definition:
One unit is defined as the amount of enzyme that will incorporate 1 nmol NTP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C.
Reaction conditions:

50 mM Tris-HCl (pH 7.5 at 25°C)
6 mM MgCl2
2 mM spermidine
10 mM DTT.

Optimal temperature: 37°C

Synthesis of unlabeled and labeled RNA that can be used:
  • For hybridization, in vitro RNA translation
  • As aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing
  • In studies of RNA secondary structure and RNA-protein interactions, RNA splicing
Storage buffer:
50 mM Tris-HCl (pH 7.5)
100 mM NaCl
20 mM 2-mercaptoethanol
0,1% Triton Х-100
50% glycerol
Storage Conditions:
Store at -20°C. Can be shipped on ice packs.
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