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chb-20003-001

Taq DNA Polymerase (free from bacterial DNA)

Taq DNA Polymerase (free from bacterial DNA)
For molecular biology. Depleted of bacterial DNA.


Price: 80.00 EUR
Not in stock
Purified from E.coli strain carrying plasmid with the cloned gene encoding Thermus aquaticus DNA polymerase. Taq DNA polymerase catalyses 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity but possesses low 5’→3’ exonuclease activity.
Unit definition:
One unit of enzyme catalyses incorporation of 10 nanomoles of deoxyribonucleotides into  acid-insoluble polynucleotide fraction in 30 min at 70°C
Applications:
  • Amplifications of DNA fragments by polymerase chain reaction (PCR)
  • DNA labelling with radionucleotides, digoxygenin or biotin
  • DNA sequencing
Tested for:absence of endo-, exodeoxyribonucleases, ribonucleases
Storage Conditions:
Store at -20°C. Can be shipped on ice packs.
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