Taq DNA Polymerase (free from bacterial DNA)

Taq DNA Polymerase (free from bacterial DNA)
For molecular biology. Depleted of bacterial DNA.

Not in stock
Purified from E.coli strain carrying plasmid with the cloned gene encoding Thermus aquaticus DNA polymerase. Taq DNA polymerase catalyses 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity but possesses low 5’→3’ exonuclease activity.
Unit definition:
One unit of enzyme catalyses incorporation of 10 nanomoles of deoxyribonucleotides into  acid-insoluble polynucleotide fraction in 30 min at 70°C
  • Amplifications of DNA fragments by polymerase chain reaction (PCR)
  • DNA labelling with radionucleotides, digoxygenin or biotin
  • DNA sequencing
Tested for:absence of endo-, exodeoxyribonucleases, ribonucleases
Storage Conditions:
Store at -20°C. Can be shipped on ice packs.
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