Purified from E.coli strain carrying plasmid with the cloned gene encoding Thermus aquaticus DNA polymerase. Taq DNA polymerase catalyses 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity but possesses low 5’→3’ exonuclease activity.
| Unit definition: |
| One unit of enzyme catalyses incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble polynucleotide fraction in 30 min at 70°C |
| Applications: |
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| Tested for:absence of endo-, exodeoxyribonucleases, ribonucleases |
| Storage Conditions: |
| Store at -20°C. Can be shipped on ice packs. |
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