Purified from E.coli strain carrying plasmid with the cloned gene encoding Thermus aquaticus DNA polymerase. Taq DNA polymerase catalyses 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity but possesses low 5’→3’ exonuclease activity.

Unit definition:

One unit of enzyme catalyses incorporation of 10 nanomoles of deoxyribonucleotides into  acid-insoluble polynucleotide fraction in 30 min at 70°C

Applications:

Amplifications of DNA fragments by polymerase chain reaction (PCR) DNA labelling with radionucleotides, digoxygenin or biotin DNA sequencing

Tested for:absence of endo-, exodeoxyribonucleases, ribonucleases

Storage Conditions:

Store at -20°C. Can be shipped on ice packs.