Uracil-DNA glycosylase (UDG) catalyzes the release of free uracil from uracil containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or less bases).
| Format: |
| 10000 units/ml in storage buffer (20 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM EDTA, 1 mM DTT, 0,1 mg/ml BSA, 50% glycerol) |
| Unit definition: |
| One unit of activity is the amount of enzyme required to release of 60pmol of uracil per minute from double-stranded, uracil-containing DNA. |
| Reaction buffer: |
| (10x) 200 mM Tris-HCl (pH8.0), 10 mM EDTA, 100 mM NaCl |
| Source: |
| Escherichia coli (E.coli) |
| Storage Conditions: |
| Store at -20°C. |
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